Virus Isolation and “Acute” West Nile Virus Encephalitis (Response to Huang et al.)

taining 300–3,000 mL of water), one thermal bottle (250 mL), one plastic bottle (50 mL), and one bucket (2,500 mL). Larvae were placed in plastic bags and transported to CIP laboratories, where they were allowed to emerge to adults during 17 days. The fourth instar larval and pupal exuvias were fixed and identified to species according to Darsie (7) and Superintendência de Campanhas de Saúde Pública (8). Twenty-five female and male Ae. albopictus from these collections are available from CIP laboratory upon request. Additional field collections are being conducted to establish the distribution range of this species along the Chiapas coastal plain, to determinate the entomologic levels of infestation, and to determine its susceptibility to insecticides. Considering the epidemiologic relevance of this discovery, we have notified the proper health authorities to take necessary control measures to reduce the possibility of increased dengue transmission and to prevent other arboviruses, such as West Nile virus (9), from being spread by this new species in southern Mexico.